Basic Lab Procedures in Clinical Bacteriology by J. Vandepitte, K. Engbaek, P. Piot, C.C. Heuck, P. Rohner

By J. Vandepitte, K. Engbaek, P. Piot, C.C. Heuck, P. Rohner

This guide is a realistic consultant, to be used via laboratory employees in healthiness centres and district hospitals, to the tactics to be in acquiring specimens, separating and making a choice on micro organism, and assessing their resistance to antibiotics. It covers bacteriological research of blood, cerebrospinal fluid, urine, stool, sputum, pharyngeal and genital specimens, and purulent exudates. specific recognition is given to the necessity for quality controls of all laboratory methods. an inventory of media and reagents wanted for the isolation and identity of the most typical bacterial pathogens is incorporated, including a sign in their relative significance for the middleman laboratory. This checklist is meant for version to neighborhood situations.

This moment variation has been up-to-date in lots of parts, together with a enormously more desirable part on stool specimens and a brand new part on serological assessments.

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Category 3: More than 105 CFU per ml. Report the count to the physician and proceed with identification and susceptibility tests if one or two different colony types of bacteria are present. These bacterial counts are strongly suggestive of UTI in all patients, including asymptomatic females. If more than two species of bacteria are present in urine samples in categories 2 and 3, report as “Probably contaminated; please submit a fresh, clean-catch specimen”. Identification Identification should be performed as rapidly as possible.

Influenzae should be tested for susceptibility to chloramphenicol using chocolate agar or a supplemented blood agar. Most ampicillin-resistant strains produce b-lactamase that can be demonstrated using one of the rapid tests recommended for the screening of potential b-lactamase-producing strains of gonococci (page 79). A 29 Urine Introduction Urine is the specimen most frequently submitted for culture. It also presents major problems in terms of proper specimen collection, transport, culture techniques, and interpretation of results.

If the reading of this test on the primary blood agar plate is inconclusive, the test should be repeated on a subculture. Colonies of H. influenzae will grow only on chocolate agar, and as satellite colonies in the vicinity of the staphylococcal streak on blood agar. Further identification may be accomplished using H. influenzae type b antiserum in the slide agglutination test. Gram-negative diplococci growing on blood and chocolate agar, and giving a rapidly positive oxidase test, may be considered to be meningococci.

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