Application of Bacterial Pigments as Colorant: The Malaysian by Wan Azlina Ahmad, Wan Yunus Wan Ahmad, Zainul Akmar Zakaria,

By Wan Azlina Ahmad, Wan Yunus Wan Ahmad, Zainul Akmar Zakaria, Nur Zulaikha Yusof

Environmental matters relating to non-stop use of man-made dyes observed a revival within the call for for usual dyes as common dyes express higher biodegradability and usually have the next compatibility with the surroundings. even if, one of many obstacles at the use of usual dyes or pigments is the low extraction yield elements (a few grams of pigment in line with kg of dried uncooked material). consequently, the exploitation of different organic resources corresponding to fungi, micro organism and mobile cultures bargains fascinating substitute. Microbial pigments akin to from bacterial origins supply the virtue by way of construction in comparison to pigments extracted from greens or animals, as a result of its basic cellphone and quickly culturing process. This publication bargains attention-grabbing perception into preliminary works conducted to illustrate the aptitude use of bacterial pigment as colorant for varied applications.

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4. From the figure, it is clearly shown that the charge delocalization is greatly enhanced by the presence of electron donating-NHR group. 0 nm Fig. 3 UV/vis spectrum of crude-purple pigment at various pH values (a) C C C O C C C O (b) NR2 HC C C O H H NR2 HC C C O H H Fig. , longer wavelength for transition (Mohan 2007). FTIR analysis of the crude-violet pigment was carried out as follows; The RWS sample (1 mg) was ground with 200 mg of KBr (spectroscopic grade) in a mortar before pressed into 10 mm diameter disks under 6 tons of pressure.

It was suggested that flexirubin reaction was shown in the presence of acid and base condition because it is one diagnostic feature of flexirubin type pigment (Balows et al. 1992). Similar finding was obtained by Kim et al. 2005, where the Flexirubin-type pigment was detected in Chryseobacterium daecheongense sp. nov. according to this method. According to Bernardet et al. 2002, since the color shift may pass unnoticed when the KOH solution is poured directly over a thin colony on an agar plate, it is strongly recommended that the test be performed on a small mass of bacterial cells collected with a loop and deposited on a glass slide placed on a white background.

US Patent 3,951,743 Shirata A, Tsukamoto T, Yasui H, Hata T, Hayasaka S, Kojima A, Kato H (2000)Isolation of bacteria producing bluish-purple pigment and use for dyeing. Japan Agric. Res. Quart 34:131–140 Song MJ, Bae J, Lee DS, Kim CH, Kim JS, Kim SW, Hong SI (2006) Purification and characterization of prodigiosin produced by integrated bioreactor from Serratia sp. KH-95. J Biosci Bioeng 101(2):157–161 Timberlake CF, Henry BS (1986) Plant pigments as natural food colours. Endeavour 10(1):31–36 Tinoi J, Rakariyatham N, Deming RL (2005)Simplex optimization of carotenoid production by Rhodotorula glutinis using hydrolyzed mung bean waste flour as substrate.

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